3T34

Arabidopsis thaliana dynamin-related protein 1A (AtDRP1A) in prefission state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-02
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	53.471, 86.407, 88.485
Unit cell angles	90.00, 90.55, 90.00

Refinement procedure

Resolution	40.307 - 2.405
R-factor	0.2389
Rwork	0.236
R-free	0.28410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x2e
RMSD bond length	0.010
RMSD bond angle	1.481
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	29759
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	160mM sodium formate, 16%(w/v) polyethylene glycol 3350, 40mM magnesium chloride, 6%(w/v) 2-Propanol, 20mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K