3T30
Human nucleoplasmin (Npm2): a histone chaperone in oocytes and early embryos
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 76 |
| Detector technology | CCD |
| Collection date | 2007-03-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.030, 107.240, 108.700 |
| Unit cell angles | 90.00, 102.54, 90.00 |
Refinement procedure
| Resolution | 40.460 - 1.900 |
| R-factor | 0.1932 |
| Rwork | 0.193 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1K5J (Xenopus nucleoplasmin) |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.659 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.265 |
| Number of reflections | 86130 | |
| <I/σ(I)> | 18.4 | 4 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 20% v/v PEG 3350, 100 mM NaCl, 25 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
