3T2X
Structure of shwanavidin low affinity mutant (F43A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9736 |
| Spacegroup name | H 3 |
| Unit cell lengths | 113.933, 113.933, 83.431 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.150 |
| R-factor | 0.14596 |
| Rwork | 0.145 |
| R-free | 0.17070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.544 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.150 |
| Number of reflections | 142657 |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 293 | 1.1M Sodium Formate, 0.05M sodium acetate, 50mM bis tris, pH 6.5, microbatch, temperature 293K |
