3T2W

Crystal structure of shwanavidin (F43A) - biotin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9736
Spacegroup name	P 43 21 2
Unit cell lengths	63.212, 63.212, 65.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.500
R-factor	0.17841
Rwork	0.177
R-free	0.20762
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.589
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.500
Number of reflections	21787
Completeness [%]	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	8.3	293	1.5M Ammonium Sulfate, 50mM tris, pH 8.3, microbatch, temperature 293K