3T2B
Fructose-1,6-bisphosphate aldolase/phosphatase from Thermoproteus neutrophilus, ligand free
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2010-08-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 112.532, 112.532, 151.126 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.790 - 1.520 |
| R-factor | 0.16573 |
| Rwork | 0.165 |
| R-free | 0.18818 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1umg |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.574 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.794 | 1.620 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.064 | 0.363 |
| Number of reflections | 74283 | |
| <I/σ(I)> | 22.4 | 6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 8% PEG3350, 0.1 M HEPES/NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
