3T24
Crystal structure of Pseudomonas aeruginosa OpdQ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-15 |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.722, 78.464, 97.519 |
| Unit cell angles | 77.76, 81.50, 70.04 |
Refinement procedure
| Resolution | 14.980 - 2.400 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qtk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.091 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 67240 | |
| <I/σ(I)> | 17.7 | 1.8 |
| Completeness [%] | 98.2 | 97.3 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 295 | 33% PEG 1K 0.2 M ammonium sulphate 50 mM Na-acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
