3T1R
MglB with tetrameric arrangement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97874 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 99.200, 118.000, 111.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.330 - 2.000 |
| R-factor | 0.18515 |
| Rwork | 0.182 |
| R-free | 0.23563 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j3w |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.078 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.330 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 43770 | |
| Completeness [%] | 98.2 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.1M MgCl2, 2.5M NaCl, 0.1 M Tris-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
