3T1Q
MglA bound to GppNHp in complex with MglB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Collection date | 2010-11-30 |
| Wavelength(s) | 0.87260 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 105.500, 175.100, 69.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.180 - 2.700 |
| R-factor | 0.2003 |
| Rwork | 0.197 |
| R-free | 0.26789 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j3w |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.889 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.180 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 17955 | |
| Completeness [%] | 99.7 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 1.25M LiCl, 0.1M Hepes, 15% PEG 6000 , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
