3T1O
MglA bound to GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97874 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.300, 47.850, 53.080 |
| Unit cell angles | 108.37, 100.10, 92.92 |
Refinement procedure
| Resolution | 45.100 - 1.900 |
| R-factor | 0.19494 |
| Rwork | 0.193 |
| R-free | 0.23203 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.055 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 34257 | |
| Completeness [%] | 96.2 | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 65% MPD, 0.1M Tris , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
