3T10
HSP90 N-terminal domain bound to ACP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2010-10-28 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.170, 44.972, 53.854 |
| Unit cell angles | 90.00, 115.15, 90.00 |
Refinement procedure
| Resolution | 26.819 - 1.240 |
| R-factor | 0.1581 |
| Rwork | 0.158 |
| R-free | 0.17200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yes |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.499 |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.240 |
| Rmerge | 0.054 |
| Number of reflections | 63893 |
| <I/σ(I)> | 42.894 |
| Completeness [%] | 97.6 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20~25% (w/v) PEG 2000 monomethyl ether, 200mM magnesium chloride, 100mM sodium cacodylate pH 6.5,, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
