3SZD
Crystal structure of Pseudomonas aeruginosa OccK2 (OpdF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-27 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.321, 206.899, 51.581 |
| Unit cell angles | 90.00, 99.09, 90.00 |
Refinement procedure
| Resolution | 14.995 - 2.311 |
| R-factor | 0.2286 |
| Rwork | 0.225 |
| R-free | 0.28050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.190 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Number of reflections | 37591 | |
| Completeness [%] | 92.8 | 90.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 25% PEG 1K 50 mM Li2SO4 50 mM Na2SO4 50 mM Na-acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
