3SZ7
Crystal structure of the Sgt2 TPR domain from Aspergillus fumigatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2010-07-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 59.610, 86.770, 116.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.397 - 1.720 |
| R-factor | 0.173 |
| Rwork | 0.169 |
| R-free | 0.20770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vyi |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.743 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.535 | 26.535 | 1.810 |
| High resolution limit [Å] | 1.720 | 5.440 | 1.720 |
| Rmerge | 0.031 | 0.502 | |
| Total number of observations | 2321 | 9471 | |
| Number of reflections | 15756 | ||
| <I/σ(I)> | 14 | 19.2 | 1.2 |
| Completeness [%] | 97.2 | 96.3 | 98.8 |
| Redundancy | 4 | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 25% PEG 1500, 0.1M MES, Malic acid, Tris, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
