3SZ6
IsdX1, an anthrax hemophore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.637, 43.659, 47.408 |
| Unit cell angles | 99.24, 96.53, 107.35 |
Refinement procedure
| Resolution | 25.574 - 1.800 |
| R-factor | 0.2113 |
| Rwork | 0.209 |
| R-free | 0.23420 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.566 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 24541 | |
| Completeness [%] | 97.2 | 94.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 298 | 2 M NaCl, 0.1 M citric acid (pH 3.5) , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
