3SZ4
Crystal Structure of LHK-Exo in complex with dAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-30 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| Unit cell lengths | 108.327, 108.327, 47.824 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.590 |
| R-factor | 0.21044 |
| Rwork | 0.208 |
| R-free | 0.26215 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3syy |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.608 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.590 | 2.600 |
| Rmerge | 0.047 | 0.473 |
| Number of reflections | 10072 | |
| <I/σ(I)> | 43.7 | 4.3 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 0.1M Tris pH 8.4, 1.0M potassium sodium tartrate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
