3SZ3

Crystal structure of Tryptophanyl-tRNA synthetase from Vibrio cholerae with an endogenous tryptophan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-11
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97903
Spacegroup name	P 21 21 2
Unit cell lengths	76.020, 94.047, 47.036
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.090 - 1.500
R-factor	0.1606
Rwork	0.160
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n9i
RMSD bond length	0.012
RMSD bond angle	1.458
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.041	0.031	0.403
Number of reflections	54037
<I/σ(I)>	19.2
Completeness [%]	99.6	98.5	98.5
Redundancy	6.7	6.7	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7		60% Tascimate, pH 7, vapor diffusion