3SY1
Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium Target OR70
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.715, 61.695, 83.234 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.501 - 1.465 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w8g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.129 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.510 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.076 | 0.833 |
| Number of reflections | 46540 | |
| <I/σ(I)> | 26.7 | 6.5 |
| Completeness [%] | 95.2 | 88.1 |
| Redundancy | 9.7 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch crystallization under oil | 5 | 291 | 20% PEG 8000, 0.1 M Manganese sulfate, 0.1 M Sodium Acetate, pH 5.0, Microbatch crystallization under oil, temperature 291K |
