3SXE
Crystal structure of AAAA+UDP+Gal with Glycerol as the cryoprotectant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-05-22 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.550, 149.630, 79.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.490 |
R-factor | 0.19173 |
Rwork | 0.190 |
R-free | 0.21938 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lz0 |
RMSD bond length | 0.029 |
RMSD bond angle | 2.256 |
Data reduction software | d*TREK (9.4SSI) |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 74.744 | 19.940 | 1.540 |
High resolution limit [Å] | 1.490 | 3.210 | 1.490 |
Rmerge | 0.043 | 0.021 | 0.364 |
Number of reflections | 50161 | ||
<I/σ(I)> | 15.5 | 51.9 | 3 |
Completeness [%] | 96.9 | 89.9 | 97.8 |
Redundancy | 4.33 | 5.09 | 3.53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | HANGING DROP | 7 | 298 | 1% PEG4000, 5% MPD, 100 mM ammonium sulfate, 70 mM sodium chloride, 50 mM ADA, pH 7.6, 30 mM sodium acetate, pH 4.6, 5 mM MnCl2, with 30% glycerol as the cryoprotectant, HANGING DROP, temperature 298K |