3SWY
CNGA3 626-672 containing CLZ domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-07-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.750, 37.650, 49.580 |
| Unit cell angles | 90.00, 110.88, 90.00 |
Refinement procedure
| Resolution | 29.217 - 1.900 |
| R-factor | 0.1981 |
| Rwork | 0.197 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zvb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.058 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.324 | 46.324 | 2.000 |
| High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
| Rmerge | 0.036 | 0.266 | |
| Total number of observations | 1086 | 2779 | |
| Number of reflections | 10016 | ||
| <I/σ(I)> | 9.6 | 12.6 | 2.5 |
| Completeness [%] | 98.6 | 97.5 | 92 |
| Redundancy | 3.1 | 3.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 20% w/v PEG3350, 200 mM potassium acetate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
