3SWK
Crystal structure of vimentin coil1B fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.150, 54.460, 44.090 |
| Unit cell angles | 90.00, 109.75, 90.00 |
Refinement procedure
| Resolution | 34.299 - 1.700 |
| R-factor | 0.2335 |
| Rwork | 0.232 |
| R-free | 0.27230 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.644 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.300 | 34.300 | 1.800 |
| High resolution limit [Å] | 1.700 | 5.060 | 1.700 |
| Number of reflections | 36328 | 1428 | 5840 |
| <I/σ(I)> | 13.26 | 32.06 | 2.59 |
| Completeness [%] | 98.9 | 96.3 | 96.1 |
| Redundancy | 4.9 | 4.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 298 | protein in 10 mM Tris pH 8, 38 mM NaCl + PEG 3350 25% w/v, 0.2M ammonium acetate, BIS-TRIS 0.1M pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
