3SWK

Crystal structure of vimentin coil1B fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97895
Spacegroup name	P 1 21 1
Unit cell lengths	40.150, 54.460, 44.090
Unit cell angles	90.00, 109.75, 90.00

Refinement procedure

Resolution	34.299 - 1.700
R-factor	0.2335
Rwork	0.232
R-free	0.27230
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.644
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.300	34.300	1.800
High resolution limit [Å]	1.700	5.060	1.700
Number of reflections	36328	1428	5840
<I/σ(I)>	13.26	32.06	2.59
Completeness [%]	98.9	96.3	96.1
Redundancy	4.9		4.64

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	298	protein in 10 mM Tris pH 8, 38 mM NaCl + PEG 3350 25% w/v, 0.2M ammonium acetate, BIS-TRIS 0.1M pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K