3SWH
Munc13-1, MUN domain, C-terminal module
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97178, 0.97945, 0.97959 |
| Spacegroup name | P 42 |
| Unit cell lengths | 160.791, 160.791, 42.239 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.119 - 2.650 |
| R-factor | 0.2469 |
| Rwork | 0.244 |
| R-free | 0.30160 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.220 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 7.190 | 2.650 |
| Rmerge | 0.075 | 0.034 | 0.674 |
| Number of reflections | 28911 | ||
| <I/σ(I)> | 10.2 | ||
| Completeness [%] | 90.0 | 76.7 | 90.7 |
| Redundancy | 4.5 | 5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 18-25% PEG400, 0.1 M MES, pH 6.0, 0.15 M sodium chloride, 10% glycerol, 5 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
