3SW8

Strep Peptide Deformylase with a time dependent dichlorobenzamide-reverse hydroxamic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-01-26
Spacegroup name	P 43
Unit cell lengths	50.015, 50.015, 91.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.125 - 1.702
R-factor	0.1955
Rwork	0.193
R-free	0.22260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.040
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.700
Number of reflections	24582
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	1.8 - 2.8 M ammonium sulfate, 1 % - 3 % PEG400, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, temperature 298K