3SW0

Structure of the C-terminal region (modules 18-20) of complement regulator Factor H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-07
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	45.970, 68.600, 77.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.970 - 1.800
R-factor	0.18394
Rwork	0.182
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oxu
RMSD bond length	0.025
RMSD bond angle	2.047
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.970	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.059	0.309
Number of reflections	23272
<I/σ(I)>	13.7	3.5
Completeness [%]	100.0	99.9
Redundancy	4.1	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	290	0.1M Sodium Acetate trihydrate, pH 4.6, 2% v/v Tacsimate, pH 4.0 (Hampton Research formulation containing: 1.8305 M Malonic acid, 0.25 M Ammonium citrate tribasic, 0.12 M Succinic acid, 0.3 M DL-Malic acid, 0.4 M Sodium acetate trihydrate, 0.5 M Sodium formate, 0.16 M Ammonium tartrate dibasic and pH adjusted using NaOH) and 16% w/v polyethylene glycol 3350 as the precipitant, VAPOR DIFFUSION, HANGING DROP, temperature 290K