3SVW
Crystal Structure of the P107V-MauG/pre-Methylamine Dehydrogenase Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-29 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 |
Unit cell lengths | 55.606, 89.001, 104.812 |
Unit cell angles | 67.05, 79.51, 79.72 |
Refinement procedure
Resolution | 33.890 - 1.860 |
R-factor | 0.13355 |
Rwork | 0.131 |
R-free | 0.17433 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l4m |
RMSD bond length | 0.025 |
RMSD bond angle | 2.085 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.5.0109) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.860 | 1.860 |
Number of reflections | 145458 | |
<I/σ(I)> | 21.1 | 7.7 |
Completeness [%] | 97.0 | 86.4 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | Drops contained 1uL protein with 3uL reservoir solution. Protein solution: 100uM P107V-MauG and 50uM preMADH in 10mM potassium phosphate pH 7.5. Reservoir solution contained: 23% w/v PEG 8000, 0.1M sodium acetate, 0.1M MES pH 6.4., VAPOR DIFFUSION, HANGING DROP, temperature 293K |