3SVO

Crystal structure of mKate mutant S158A/S143C at pH 10.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	140
Detector technology	CCD
Collection date	2010-02-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.978
Spacegroup name	I 41
Unit cell lengths	161.705, 161.705, 75.668
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.581 - 1.984
R-factor	0.1729
Rwork	0.170
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.080
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.060
High resolution limit [Å]	1.980	1.980
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10	298.15	20% PEG3350, 0.1M glycine, 0.2M MgCl2, pH 10.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K