3SVD
Engineered medium-affinity halide-binding protein derived from YFP: bromide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9199 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.520, 62.660, 65.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.530 - 1.780 |
| R-factor | 0.1681 |
| Rwork | 0.167 |
| R-free | 0.19600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3svc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.297 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.530 |
| High resolution limit [Å] | 1.780 |
| Number of reflections | 20909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | 21% PEG2000, 50 mM sodium acetate, 90 mM magnesium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
