3SVB
Engineered medium-affinity halide-binding protein derived from YFP: fluoride complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1109 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.914, 63.194, 69.229 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.673 - 1.300 |
R-factor | 0.1374 |
Rwork | 0.136 |
R-free | 0.16380 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.384 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.320 |
High resolution limit [Å] | 1.300 | 3.530 | 1.300 |
Rmerge | 0.040 | 0.023 | 0.409 |
Number of reflections | 54466 | ||
<I/σ(I)> | 15.5 | ||
Completeness [%] | 97.8 | 98.1 | 76.2 |
Redundancy | 6.6 | 6.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 290 | 10% PEG3000, 150 mM ammonium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 290K |