3SVA
Crystal structure of V57D mutant of human cystatin C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 140.145, 140.145, 140.145 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.460 - 3.020 |
| R-factor | 0.20309 |
| Rwork | 0.201 |
| R-free | 0.24191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g96 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.623 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.130 | |
| Number of reflections | 4864 | |
| <I/σ(I)> | 23.3 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.9 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Imidazole pH 6.5, 0.1M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
