3SUZ

Crystal structure of Rat Mint2 PPC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	111.662, 111.662, 84.586
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.830 - 2.700
R-factor	0.265
Rwork	0.265
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aqc
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.830	2.750
High resolution limit [Å]	2.700	2.700
Number of reflections	15588
Completeness [%]	91.4	91.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1M MES, 38% P400 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K