3SUK
Crystal structure of cerato-platanin 2 from M. perniciosa (MpCP2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-31 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.45860 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.460, 85.960, 43.560 |
| Unit cell angles | 90.00, 106.82, 90.00 |
Refinement procedure
| Resolution | 14.500 - 1.340 |
| R-factor | 0.17484 |
| Rwork | 0.173 |
| R-free | 0.20505 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3suj |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.698 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.410 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.065 | 0.270 |
| Number of reflections | 43181 | |
| <I/σ(I)> | 22.2 | 8.9 |
| Completeness [%] | 92.4 | 87.7 |
| Redundancy | 8.3 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.1 M sodium acetate pH 4.6, 8% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
