3SUB
Crystal structure of the catalytic domain of Plasmodium falciparum ARF GTPase activating protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-01-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.26966, 1.2828, 1.2834 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 95.900, 95.900, 92.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.810 - 2.400 |
R-factor | 0.2095 |
Rwork | 0.209 |
R-free | 0.22696 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.900 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 19650 | |
<I/σ(I)> | 8.8 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 4 mM zinc chloride, 1.4 M lithium sulfate, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |