3STZ

KcsA potassium channel mutant Y82C with nitroxide spin label

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Detector technology	CCD
Collection date	2008-12-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	155.732, 155.732, 76.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.500
Rwork	0.246
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.500
Number of reflections	29442
Completeness [%]	92.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K