3STY
Crystal Structure of tomato Methylketone Synthase I T18A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.292, 106.182, 59.847 |
| Unit cell angles | 90.00, 96.63, 90.00 |
Refinement procedure
| Resolution | 37.100 - 1.700 |
| R-factor | 0.2007 |
| Rwork | 0.196 |
| R-free | 0.21340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7yas |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.100 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 64470 | |
| <I/σ(I)> | 23.8 | 2 |
| Completeness [%] | 98.0 | 96 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1M MOPSO-Na+, 17.0% (w/v) PEG 8000, 0.3M NaBr, 2mM dithiothreitol, vapor diffusion, hanging drop, temperature 277K |
