3STR

Strep Peptide Deformylase with a time dependent thiazolidine hydroxamic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Detector technology	CCD
Collection date	2008-11-28
Detector	RAYONIX MX-300
Wavelength(s)	0.9787
Spacegroup name	P 43
Unit cell lengths	50.274, 50.274, 91.486
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.074 - 1.750
R-factor	0.1952
Rwork	0.192
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.054
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.750
Number of reflections	22946
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	0.1 M HEPES, 3.3 M AmSO4, 3 % PEG 400, pH 7.5, VAPOR DIFFUSION, temperature 298K