3STQ
Hypothetical protein PA2703 Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 91.897, 113.265, 143.467 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.899 - 2.284 |
| R-factor | 0.2095 |
| Rwork | 0.208 |
| R-free | 0.23790 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.096 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.900 | 2.420 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.082 | 0.627 |
| Number of reflections | 34226 | |
| <I/σ(I)> | 18.69 | 3.31 |
| Completeness [%] | 99.5 | 97.4 |
| Redundancy | 7.73 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 295 | 100mM Citric acid, 16%(v/v) MPD, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
