3STH

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Toxoplasma gondii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-21
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	103.367, 104.322, 146.625
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.250
R-factor	0.175
Rwork	0.172
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cps
RMSD bond length	0.011
RMSD bond angle	1.277
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.290
High resolution limit [Å]	2.250	2.250
Rmerge	0.120	0.481
Number of reflections	75704
<I/σ(I)>	5.7
Completeness [%]	99.7	98.7
Redundancy	5.6	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	TOGOA.00914.A.A1.PW28008 23.8 MG/ ML, PH 5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K, PACT F11, 0.2 M Sodium citrate, 0.1M Bis Tris propand pH 6.5, 20% PEG 3350