3SSU

Crystal structure of vimentin coil1A/1B fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9786
Spacegroup name	I 21 21 21
Unit cell lengths	45.600, 83.497, 118.606
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.293 - 2.603
R-factor	0.2403
Rwork	0.237
R-free	0.27190
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.097
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.640
High resolution limit [Å]	2.600	7.030	2.600
Number of reflections	7261
<I/σ(I)>	8.9
Completeness [%]	99.9	98	100
Redundancy	5.5	4.8	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.04M calcium acetate, 0.1M MES pH 6, isopropanol 6%, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.04M calcium acetate, 0.1M MES pH 6, isopropanol 6%, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.04M calcium acetate, 0.1M MES pH 6, isopropanol 6%, VAPOR DIFFUSION, HANGING DROP, temperature 277K