3SSR
CcmK2 dodecamer - form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97949 |
Spacegroup name | P 6 |
Unit cell lengths | 74.050, 74.050, 66.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.483 - 1.600 |
R-factor | 0.1555 |
Rwork | 0.153 |
R-free | 0.17820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ssq |
RMSD bond length | 0.006 |
RMSD bond angle | 0.893 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 27433 | |
<I/σ(I)> | 16.1 | 5 |
Completeness [%] | 99.2 | 99.9 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 293 | 25% PEG 4000, 0.2 M (NH4)2SO4 and 15% glycerol , pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 293K |