3SSF
Crystal structure of RNA:DNA dodecamer corresponding to HIV-1 polypurine tract, at 1.6 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-04 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 41.904, 41.904, 57.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.320 - 1.600 |
| R-factor | 0.186 |
| Rwork | 0.181 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pjo |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.258 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.550 |
| Number of reflections | 8012 | |
| <I/σ(I)> | 41.7 | 2.851 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 6.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 292 | 40% MPD, 0.04M hexamminecobalt(III) chloride, 0.02M magnesium chloride, 0.04M lithium chloride, 0.04M sodium cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
