3SSD
DNA binding domain of restriction endonuclease bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | FLAT PANEL |
| Collection date | 2008-12-05 |
| Detector | MAR555 |
| Wavelength(s) | 0.81500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.085, 67.189, 142.414 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.020 - 2.200 |
| R-factor | 0.20199 |
| Rwork | 0.196 |
| R-free | 0.26221 |
| Structure solution method | MR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.353 |
| Data reduction software | MOSFLM (6.2.6) |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | MOLREP (9.2.10) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.030 | 29.030 | 2.320 |
| High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
| Rmerge | 0.070 | 0.054 | 0.254 |
| Number of reflections | 18339 | ||
| <I/σ(I)> | 18.8 | 7.8 | 1.2 |
| Completeness [%] | 99.8 | 96.8 | 99.9 |
| Redundancy | 5.6 | 4.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 6.8 | 292 | 0.2 M LiCl, 18% PEG4000, pH 6.8, sitting drop, temperature 292K |
