3SS8
Crystal structure of NFeoB from S. thermophilus bound to GDP.AlF4- and K+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.346, 75.401, 156.494 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.510 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lx5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.468 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.122 | 0.050 | 0.483 |
| Number of reflections | 20085 | ||
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 99.5 | 97.4 | 97.7 |
| Redundancy | 4.1 | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M PCB buffer, 25%(w/v) PEG 1500, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
