3SRY
Engineered high-affinity halide-binding protein derived from YFP: halide-free
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.233, 62.506, 69.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.530 - 1.159 |
| R-factor | 0.1322 |
| Rwork | 0.131 |
| R-free | 0.15710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sst |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.684 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.530 | 50.000 | 1.180 |
| High resolution limit [Å] | 1.159 | 3.150 | 1.160 |
| Rmerge | 0.053 | 0.038 | 0.450 |
| Number of reflections | 73705 | ||
| <I/σ(I)> | 16.9 | ||
| Completeness [%] | 94.2 | 98.9 | 58 |
| Redundancy | 6.5 | 6.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 290 | 17% PEG3000, 150 mM sodium acetate, 90 mM magnesium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
