3SRI
Crystal structure of Plasmodium falciparum AMA1 in complex with a 29aa PfRON2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2011-02-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.95367 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.724, 38.137, 72.078 |
| Unit cell angles | 90.00, 97.72, 90.00 |
Refinement procedure
| Resolution | 35.040 - 1.600 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.19480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z40 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.970 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.056 | 0.618 |
| Number of reflections | 48207 | |
| <I/σ(I)> | 12.7 | 1.7 |
| Completeness [%] | 94.9 | 92.8 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 290 | 20% PEG 4000, 0.1M Tris/HCL, 0.1M sodium acetate, 20% isopropanol, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
