3SQP
Structure of human glutathione reductase complexed with pyocyanin, an agent with antimalarial activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.080, 67.370, 78.860 |
| Unit cell angles | 68.98, 66.81, 62.54 |
Refinement procedure
| Resolution | 37.710 - 2.210 |
| R-factor | 0.146 |
| Rwork | 0.144 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3djj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.903 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.710 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.210 |
| Number of reflections | 47828 | |
| Completeness [%] | 93.9 | 78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 660 MM AMMONIUM SULFATE, 100 MM POTASSIUM PHOSPHATE, PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295.0K |
