3SQJ
Recombinant human serum albumin from transgenic plant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.610, 38.368, 184.037 |
| Unit cell angles | 90.00, 104.93, 90.00 |
Refinement procedure
| Resolution | 47.800 - 2.050 |
| R-factor | 0.238 |
| Rwork | 0.235 |
| R-free | 0.30260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bj5 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.852 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.800 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 82055 |
| <I/σ(I)> | 24.4 |
| Completeness [%] | 99.6 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 28% PEG 3350, 50mM phosphate, 150 mM KCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
