3SQB

Structure of the major type 1 pilus subunit FimA bound to the FimC chaperone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	90.6
Detector technology	CCD
Collection date	2006-09-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.071
Spacegroup name	P 21 21 2
Unit cell lengths	79.620, 142.180, 171.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.386 - 3.200
R-factor	0.2617
Rwork	0.261
R-free	0.30040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bwu
RMSD bond length	0.005
RMSD bond angle	0.827
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000		3.300
High resolution limit [Å]	3.200	50.000	3.200
Rmerge	0.138	0.079	0.769
Number of reflections	32913	5	2840
<I/σ(I)>	10.89	10.67	2.34
Completeness [%]	99.8	27.8	99.9
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.1 M sodium acetate, 0.2 M magnesium chloride, 13% PEG 4000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K