3SQB
Structure of the major type 1 pilus subunit FimA bound to the FimC chaperone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90.6 |
| Detector technology | CCD |
| Collection date | 2006-09-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.071 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 79.620, 142.180, 171.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.386 - 3.200 |
| R-factor | 0.2617 |
| Rwork | 0.261 |
| R-free | 0.30040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bwu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.827 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.300 | |
| High resolution limit [Å] | 3.200 | 50.000 | 3.200 |
| Rmerge | 0.138 | 0.079 | 0.769 |
| Number of reflections | 32913 | 5 | 2840 |
| <I/σ(I)> | 10.89 | 10.67 | 2.34 |
| Completeness [%] | 99.8 | 27.8 | 99.9 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.1 M sodium acetate, 0.2 M magnesium chloride, 13% PEG 4000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
