3SQ6

Crystal Structures of the Ligand Binding Domain of a Pentameric Alpha7 Nicotinic Receptor Chimera with its Agonist Epibatidine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	81.237, 141.069, 130.207
Unit cell angles	90.00, 99.65, 90.00

Refinement procedure

Resolution	47.470 - 2.800
R-factor	0.234
Rwork	0.234
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.470
High resolution limit [Å]	2.800
Rmerge	0.111
Number of reflections	61567
Completeness [%]	86.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION			vapor diffusion