3SPE

Crystal structure of the tail sheath protein protease resistant fragment from bacteriophage phiKZ

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97948, 0.97962, 0.94949
Spacegroup name	P 21 21 2
Unit cell lengths	70.892, 156.715, 61.003
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.424 - 2.400
R-factor	0.2579
Rwork	0.256
R-free	0.28650
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.294
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.490
High resolution limit [Å]	2.400	5.160	2.400
Rmerge	0.107	0.059	0.646
Number of reflections	26951
Completeness [%]	98.6	99.5	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	1.8 M Na/K Phosphate, pH 5.0 with 0.1 M Na Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K