3SOZ
Cytoplasmic Protein STM1381 from Salmonella typhimurium LT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791827 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.387, 92.387, 240.904 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.260 - 2.600 |
| R-factor | 0.19598 |
| Rwork | 0.192 |
| R-free | 0.26480 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.874 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.620 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 33198 | |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.1 M Acetate pH 4.5 0.8 M NaH2PO4/1.2 M K2HPO4 (Wizard 2, screen 35), VAPOR DIFFUSION, SITTING DROP, temperature 289.0K |
