3SOY

Nuclear transport factor 2 (NTF2-like) superfamily protein from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97931, 0.97918
Spacegroup name	I 41 2 2
Unit cell lengths	79.346, 79.346, 107.293
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.110 - 2.000
R-factor	0.17795
Rwork	0.176
R-free	0.22085
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.742
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.110	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.079	0.060	0.787
Number of reflections	11364
<I/σ(I)>	8.1
Completeness [%]	95.3	96.9	78.3
Redundancy	13.3	11.8	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	1.1M Sodium Malonate pH 7.0, 0.1M HEPES:NaOH pH 7.0, 0.5% Jeffamine ED-2001 pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K