3SOO

Crystal structure of a LINE-1 type transposase domain-containing protein 1 (L1TD1) from HOMO SAPIENS at 2.73 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-21
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97932,0.97907
Spacegroup name	P 65 2 2
Unit cell lengths	69.140, 69.140, 292.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.712 - 2.730
R-factor	0.2275
Rwork	0.226
R-free	0.25930
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.712	48.712	2.880
High resolution limit [Å]	2.730	8.630	2.730
Rmerge		0.028	0.598
Total number of observations		2182	11721
Number of reflections	11913
<I/σ(I)>	15.2	22.5	1.3
Completeness [%]	99.8	96.7	99.9
Redundancy	6.7	4.8	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	0.2M potassium sodium tartrate, 2.0M ammonium sulfate, 0.1M sodium citrate pH 5.6, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 277K